High-Throughput SISCAPA-Based Peptide Quantitation Using an Agilent RapidFire High-Throughput Mass Spectrometry System
نویسنده
چکیده
A high-throughput SISCAPA workfl ow has been developed that uses RapidFire/MS technology to enable sensitive quantitation of peptides from plasma digests with a sample cycle time of approximately 10 seconds. The optimized system includes capture of target proteotypic peptides using rigorously selected, high affi nity antipeptide monoclonal antibodies and reduction of background peptides using a novel treatment with magnetic bead immunoadsorbents. The method can be multiplexed, thus facilitating analysis of large sample sets for biomarker analysis in a fraction of the time of other methods. Authors Leigh Anderson SISCAPA Assay Technologies Rockville, MD USA Morteza Razavi, Matt Pope, and Terry Pearson University of Victoria Biochemistry Department Canada Lauren Frick, Christine Miller, and William LaMarr Agilent Technologies, Inc. Wakefi eld, MA USA High-Throughput SISCAPA-Based Peptide Quantitation Using an Agilent RapidFire High-Throughput Mass Spectrometry System
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